Proteomic identification of cryostress in epididymal spermatozoa
نویسندگان
چکیده
منابع مشابه
Proteomic changes in mammalian spermatozoa during epididymal maturation.
Epididymal maturation is associated with the activation of a cAMP-induced tyrosine phosphorylation cascade, which is ultimately associated with the expression of capacitation-dependent sperm functions, such as hyperactivated movement and acrosomal exocytosis. As spermatozoa progress through the epididymis they first acquire the capacity to phosphorylate tyrosine on targets on the principal piec...
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Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin bl...
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This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then...
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The first report of a foal born after artificial insemination (AI) with frozen/thawed semen, more than fifty years ago, was the result of insemination with epididymal spermatozoa (Barker en Gandier, 1957). Ever since, reports have confirmed pregnancies and/ or births after AI with epididymal semen, which proves the fertilizing capacity of epididymal semen (Morris, 2002; Monteiro et al., 2011). ...
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Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur o...
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ژورنال
عنوان ژورنال: Journal of Animal Science and Biotechnology
سال: 2016
ISSN: 2049-1891
DOI: 10.1186/s40104-016-0128-2